CLOSE

Federica Facciotti

  • RESEARCH AREA

    1. My research group specifically focuses on understanding and functionally manipulating for therapeutic purposes the interactions between the mucosal immune system and the intestinal microenvironment.

    The intestinal compartment is a complex biological system composed by different type of cells (immune cells, epithelial cells, gut microbiota) involved in functional crosstalks aimed at maintaining a balance between tolerance and immunity. These interactions may give rise to different functional outcomes. In healthy conditions immune cells contribute to intestinal homeostasis maintenance, while in genetically predisposed individuals hyperactivation of immune cells may lead to in chronic autoimmune intestinal inflammation.  In the context of intestinal tumours, defective activation of immune cells may contribute to decreased immunesurveillance and tumour development.

    Specifically, our projects focus at:

    • deciphering the role of conventional and unconventional intestinal CD4+ T helper cells in contributing to tissue homeostasis and in participating to inflammatory immune responses;
    • understanding the functions of intestinal T lymphocytes in the control of epithelial neoplastic transformations;
    • dissecting the functional interactions between T cells , the gut microbiota and the intestinal microenvironment during intestinal neoplastic transformation and inflammation
    • manipulating the function of immune cells for therapeutic purposes.

     To do so, we take advantage of a translational approach involving in vitro systems, murine models of colorectal cancer and intestinal inflammation, and patients’-derived surgical specimens.

     

    Clinical trials:

    1. Study on the role of gut microbiota in the shaping of iNKT cell function during colorectal cancer (CRC) progression, IEO 0566
    2. Modulazione funzionale del sistema immunitario mucosale attraverso il trapianto di microbiota fecale (FMT): uno studio traslazionale (IMMUBAC) (with Policlinico Hospital Milan and Policlinico Hospital Tor Vergata, Rome).

     

    Group Members:

    Technician
    Erika Mileti

    Undergraduates
    Angelika Diaz Besabe

    PhD Students
    Georgia Lattanzi

    Post-Doc
    Claudia Burrello

     

  • PUBLICATIONS

    • Burrello C, Pellegrino G, Giuffrè MR, Lovati G, Magagna I, Bertocchi A, Cribiù FM, Boggio F, Botti F, Trombetta E, Porretti L, Di Sabatino A, Vecchi M, Rescigno M, Caprioli F, Facciotti F.
      “Mucosa-associated microbiota drives pathogenic functions in IBD-derived intestinal iNKT cells”
      Life Science Alliance, accepted 1st February 2019
    • Burrello C, Garavaglia F, Cribiù FM, Ercoli G, Lopez G, Troisi J, Colucci A, Guglietta S, Carloni S, Guglielmetti S, Taverniti V, Nizzoli G, Bosari S, Caprioli F, Rescigno M, Facciotti F.
       “Therapeutic fecal microbiota transplantation controls intestinal inflammation through IL10 secretion by immune cells”
      Nature Communications 2018 Dec 5;9(1):5184. doi: 10.1038/s41467-018-07359-8.
    • Cribiù FM, Burrello C, Tacchi R, Boggio F, Ricca D, Caprioli F, Bosari S, Facciotti F.
      “Using robotic systems to process and embed murine samples for histological analyses”.
      J Vis Exp. 2019 Jan 7;(143). doi: 10.3791/58654.
    • Bevivino G, Sedda S, Franzè E, Stolfi C, Di Grazia A, Dinallo V, Caprioli F, Facciotti F, Colantoni A, Ortenzi A, Rossi P, Monteleone G.
      Follistatin-like protein 1 sustains colon cancer cell growth and survival.
      Oncotarget. 2018 Jul 27;9(58):31278-31290. doi: 10.18632/oncotarget.25811. eCollection 2018 Jul 27.
    • Burrello C, Garavaglia F, Cribiù FM, Ercoli G, Bosari S, Caprioli F, Facciotti F
      “Short-term oral antibiotics treatment promotes inflammatory activation of colonic invariant natural Killer and Conventional CD4+ T cells”
      Frontiers in medicine Published on 07 February 2018; doi: 10.3389/fmed.2018.00021
    • Nizzoli G, Burrello C, Cribiù FM, Lovati G, Ercoli G, Botti F, Trombetta E, Porretti L, Todoerti K, Neri A, Giuffrè MR, Geginat J, Vecchi M, Rescigno M, Paroni M, Caprioli F*, Facciotti F*.
      “Pathogenicity of in-vivo generated intestinal Th17 lymphocytes is IFNγ dependent.” Journal of Crohn’s and Colitis 2018 Jul 30;12(8):981-992. doi: 10.1093/ecco-jcc/jjy051
    • Cribiù FM, Burrello C, Ercoli G, Garavaglia F, Villanacci V, Caprioli F, Bosari S, Facciotti F.
      “Implementation of an automated inclusion system for the histological analysis of murine models of intestinal inflammation: a feasibility study in DSS-induced chronic colitis”.
      European Journal of Inflammation, 2018 Volume 16-1

     

PARTNERSHIP

Università degli Studi di Milano Ecancer Medical Science IFOM-IEO Campus

CREDITS

Ministero della Salute Joint Commission International Breastcertification bollinirosa

© 2013 Istituto Europeo di Oncologia - via Ripamonti 435 Milano - P.I. 08691440153 - Codice intermediario fatturazione elettronica: A4707H7

IRCCS - ISTITUTO DI RICOVERO E CURA A CARATTERE SCIENTIFICO